产品货号：
Z31079
中文名称：
TFAM抗体
英文名称：
Anti-TFAM Antibody (Clone#4D9)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-TFAM Antibody (Clone#4D9) |
| 指标别名 | Cell and organelle markers;Mitochondria Marker;Mitochondrial Marker;Mitochondrion Marker;MtTF1;mtTFA;TCF 6;TCF6;TCF6L1;TCF6L2;TCF6L3;TFAM;Transcription factor 6;Transcription factor 6 like 2 |
| 克隆性 | Monoclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,FCM |
| 基因名称 | TFAM |
| 克隆号 | 4D9 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2b |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA(214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY),different from the related mouse and rat sequences by five amino acids. |
| 实际分子量 | 24KD |
| 成分 | 200μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 200μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | TFAM(Transcription factor A,mitochondrial),also known as TCF6 or TCF6L2,is a 162-amino acid protein that activates transcription of each mitochondrial DNA(mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters.By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines,Milatovich et al.(1992) mapped TFAM sequences,which they called MTTF1,to 3 different chromosomes:chromosomes 10,7p,and 11q.By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization,Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1,or TCF6L3) are pseudogenes.Larsson et al.(1997) mapped the mouse mitochondrial transcription factor A gene(Tfam) to the central part of mouse chromosome 10.This region exhibits syntenic homology with human 10q21.Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals.It also has a role in mitochondrial DNA replication,since transcription generates an RNA primer necessary for initiation of mtDNA replication. |
| Uniprot ID | Q00059 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1866) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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